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QuikChange® II Site-Directed Mutagenesis Kits         更多点突变
试剂盒

Applications

        Generate point mutations

        Delete or insert single or multiple amino acids

        Ideal for use with a wide range of plasmid sizes <8 kb

        Can be used for domain swapping experiments

 

Delivering Enhanced Performance

QuikChange® II site-directed mutagenesis kits** represent the next evolution in the QuikChange product line. These kits offer enhanced performance by providing the highest accuracy available. Produced with only the highest quality reagents, QuikChange II kits deliver greater than 80% efficiency and the best performance possible for every site-directed mutagenesis application.

 

Drastically Reduce Unwanted Errors

In the QuikChange® II kits, high accuracy is achieved through the use of our high-fidelity DNA polymerase, which virtually eliminates second-site errors, and a unique non-PCR method which prevents propagation of amplified errors.

 

Kit Method Delivers Greater Than 80% Efficiency

QuikChange® II kits are a fast, easy and efficient non-PCR alternative to other site-directed mutagenesis techniques. The QuikChange II kit strategy uses a unique method that does not require single-stranded DNA or labor intensive, difficult-to-perform steps. Cesium chloride-purified or miniprep DNA (such as plasmid DNA purified with the StrataPrep® plasmid miniprep purification kit) can be mutated and transformed with 80-100% of the resulting colonies containing the desired mutations.

 

Simple Non-PCR Procedure

Unlike PCR-based mutagenesis, the QuikChange II method relies on linear amplification of template DNA by mutagenesis-grade high fidelity DNA polymerase. Mutagenic primers are extended during temperature cycling, incorporating the mutation of interest into the newly synthesized strands.1 The unique primer design allows replication of only the parental strand to take place in each successive round. Because our high-fidelity DNA polymerase is able to replicate DNA with the highest accuracy compared to other thermostable enzymes, it virtually eliminates the possibility of producing undesirable random errors. Final treatment with Dpn I ensures the digestion of only the dam-methylated parental strands.2 The resulting nicked circular mutagenic strands are transformed into the XL-1 Blue supercompetent cells, where bacterial ligase repairs the nick and allows normal replication to occur. Greater than 80% of the colonies contain the desired insert. The QuikChange II kit is recommended for plasmids <8kb while the QuikChange II XL Kit is ideal for high-efficiency mutagenesis of plasmids larger than 8kb in a single day.

 

 


应用领域

产品名称

主要特性

货号

规格

价格

(元)

说明书

下载

备注

  

 

QuikChange Lightning Mutagenesis Kit

质粒<14kb

210518 推荐

10T

4592

点击

现货8

QuikChange Lightning Mutagenesis Kit

质粒<14kb

210519 推荐

30T

12260

点击

现货8

QuikChange Site-Directed Mutagenesis Kit

质粒<8kb

200518

30T

11987

点击

来电询问

QuikChange Site-Directed Mutagenesis Kit

质粒<8kb

200519

10T

4223

点击

来电询问

QuikChange II Site-Directed Mutagenesis Kit

质粒<8kb

200523

10T

4203

点击

来电询问

QuikChange II Site-Directed Mutagenesis Kit

质粒<8kb

200524

30T

11190

点击

来电询问

QuikChange XL Site-Directed Mutagenesis Kit

质粒>8kb

200516

30T

13255

点击

来电询问

QuikChange XL Site-Directed Mutagenesis Kit

质粒>8kb

200517

10T

4915

点击

现货8

QuikChange II XL Site-Directed Mutagenesis Kit

质粒>8kb

200521

10T

4787

点击

来电询问

QuikChange II XL Site-Directed Mutagenesis Kit

质粒>8kb

200522

30T

12591

点击

来电询问

QuikChange Lightning Multi Site-Directed Mutagenesis Kit

多达5个点

210515推荐

10T

6481

点击

现货8

QuikChange Lightning Multi Site-Directed Mutagenesis Kit

多达5个点

210513推荐

30T

17300

点击

现货8

QuikChange Multi Site-Directed Mutagenesis Kit

多达5个点

200514

30T

18659

点击

来电询问

QuikChange Multi Site-Directed Mutagenesis Kit

多达5个点

200515

10T

6983

点击

来电询问

随机

突变

GeneMorph II Random Mutagenesis Kit 随机突变克隆 200550 10T 8310

点击

来电询问

GeneMorph II EZClone Domain Mutagenesis Kit   趋错随机突变 200552 10T 6110

点击

来电询问

Dpn I Restriction E500402.pdfnzyme

配套试剂

500402

200U

1848

点击

来电询问


    体外定点突变技术是研究蛋白质结?#36141;?#21151;能之间复杂关系的有力工具,也是实验室中改造和优化基因常用的手段,对某个已知基因的特定碱基进行定点改变、缺失或者插入可以改变对应氨基酸序列和蛋白质结构,可以进一步了解蛋白质结?#36141;?#21151;能之间的关系,探讨蛋白质的结构域。上海前尘公司常备著名品牌Stratagene的各种定点突变检测试剂盒,并提供专业的技术支持,使您科研无忧!

推荐使用翊圣生物公司的相关产品:可与stratagene品质媲美!

产品名称

货号

规格

价格

Hieff MutTM Site-Directed Mutagenesis Kit 定点突变试剂盒

11003ES10

10 rxn

720

Hieff MutTM Multi Site-Directed Mutagenesis Kit 多点突变试剂盒

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Hieff CloneTM Multi One Step Cloning Kit

多片段一步法(快速/无缝)克隆试剂盒

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10906 ES50

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Hieff CloneTM One Step Pcr Cloning Kit

一步法(快速/无缝)克隆试剂盒

10905ES25

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432

10905ES62

5×25T

1944

 

 

 

 

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